The test kit is detecting for the quantitative analysis of Sarafloxacin in honey, animal tissue (chicken, pork, fish, and shrimp), eggs, milk and milk powder.
Principle of test
The Sarafloxacin (SAR) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The Sarafloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the Sarafloxacin-horseradish peroxidase (Sarafloxacin -HRP) conjugate. If the Sarafloxacin residue is present in the sample, it will compete for the Sarafloxacin antibody, thereby preventing the Sarafloxacin-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples A value is an inverse relationship with Sarafloxacin residue content. Lastly, compared with the standard curve can be concluded the Sarafloxacin residues in sample.
Kit sensitivity: 0.1ppb(ng/ml)
reactive mode: 25℃, 45min～15min
The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.