Trimethoprim (TMP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trimethoprim in Compound feed, tissue, serum, urine, and etc.
Principle of Test
The Trimethoprim (TMP) ELISA Test Kit is using indirect competitive enzyme immunoassay method.The Trimethoprim coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, Trimethoprim of samples will compete with coated Trimethoprim coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with Trimethoprim residue content. Lastly, compared with the standard curve can be concluded the Trimethoprim residues in sample.
Kit sensitivity: 0.03 ppb(ng/ml)
reactive mode: 25℃, 45min～15min
The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.